Supercritical fluid chromatography (SFC) uses supercritical CO2 as the mobile phase characterized by low polarity like n-hexane, lower viscosity, and high diffusion coefficient which offers potential for advanced separations that are efficient when compared to HPLC-based methods. SFC enables unique analyses, such as high-resolution chromatography, simultaneous analysis of compounds over a wide polarity range, and improvement of MS detection sensitivity. Stable baseline with low pulsation is realized by LC-30ADSF with a built-in cooler for pump heads, which delivers mobile phases up to 5 mL/min at pressures up to 66 MPa. The low dead volume of SFC-30A (0.7 μL) allows a mass spectrometer to be directly connected to the SFC system without splitting so that higher sensitivity can be achieved.
Improved separation and detection capabilities result from the low viscosity and high diffusion coefficient of supercritical CO2. Isomeric compounds normally difficult in HPLC, are easily separated using SFC based systems.
As supercritical CO2 has properties similar to hexane, SFC is well-suited for analyzing compounds with different polarities, providing an ideal solution for the simultaneous analysis of fatty acids and glycerides.
Nexera UC combines on-line supercritical fluid extraction (SFE) and SFC in a single flow path (patented technology). Target compounds are extracted from solid samples and then automatically transferred to SFC analytical system for data acquisition. In addition, off-line SFE can simplify pretreatment over existing solvent extraction methods. As against a typical sample preparation takes 35 minutes requiring several manual steps, Nexera UC, on-line SFE/SFC analysis does the processing in as little as 5 minutes with only a few simple sample preparation steps. Supercritical Fluid Extraction Unit SFE-30A, operates at a maximum temperature of 80℃ to allow faster and more complete extractions. Five types of extraction vessels (two sizes (5 mL, 0.2 mL)made of PEEK(maximum pressure 20MPa), three sizes (10 mL, 5 mL, 0.2 mL) made of SUS) can be chosen based on the sample and sample amount.
With a reduced organic solvent consumption, Nexera UC significantly contributes to sustainable analysis with reduced load on environment.
The Nexera UC chiral screening system can significantly improve screening efficiency during separation of optical isomers / simultaneous analysis of multiple analytes by automatically switching between up to 12 columns and 7 types of modifiers, and can even adjust the blending ratio for those conditions. Furthermore, by using a UHPLC/SFC switching system, screening can successively switch between UHPLC and SFC analysis modes.
