Next-Gen Protein Sequencing - Spincotech

Optimizing Immunoprecipitation Workflows for Next-gen Protein Sequencing

Tech Note

Next-gen protein sequencing on the Platinum® Pro platform enables detailed protein characterization, but performance depends on optimized IP workflows, with this study showing that common low-pH elution buffers reduce alignment and peptide identification efficiency.

Next-Gen Protein Sequencing

Quantum-Si enables next-generation single-molecule protein sequencing through the Platinum® Pro benchtop system, delivering real-time protein identification and variant analysis in a compact, user-friendly workflow.

Learn About Next-Gen Protein Sequencing Technology

How it Works

01. Prepare

Library Preparation and Sequencing Kits contain everything you need to initiate the next-gen protein sequencing process, from sample prep to loading.

Includes reagents to digest and functionalize proteins
Supplies aminopeptidases and recognizers to begin sequencing

02. Sequence

Platinum® Pro sequences peptides by capturing fluorescent signals from each N-terminal amino acid (NAA) binding event, enabling real-time, single-molecule protein sequencing.

Uses aminopeptidases to sequentially cleave each amino acid
Repeats the process to sequence the entire peptide

03. Analyze

Sequencing data is automatically uploaded to the Platinum Analysis software, which interprets results at the single-molecule level. No bioinformatics expertise is required

Delivers clear, interpretable single-molecule protein insights
Removes the need for complex data analysis tools

Single-molecule Protein Sequencing Applications

Protein Barcoding

Simultaneously characterize multiple protein variants with barcoding, increasing throughput and improving efficiency in screening and therapeutic development.

Protein Identification

Achieve single-amino acid resolution for precise protein characterization, and validation in research and diagnostics.

Protein Identification

Detect amino acid substitutions and isobaric variations with single-molecule resolution, uncovering critical insights into health and disease.

Post-translational Modification Analysis

Precisely analyze proteoforms and post-translational modifications (PTM) to gain deeper functional insights into protein regulation and activity.

Antibody Sequencing and Characterization

Gain deep insights into antibody specificity, affinity, and purity. This advanced approach ensures precise targeting and maximized efficacy in therapeutic development.

Two Exciting New Papers Demonstrate the Value of Single-molecule Protein Sequencing in Clinical Proteomics and Pathogen/Toxin Detection

A new peer-reviewed paper in the journal Analytical Chemistry from Stanford University and a new preprint on Chemrxiv from the U.S. Naval Research Laboratory illustrate how single-molecule protein sequencing is advancing clinical research and complex real-world problems.

Our Expertise

Next-Gen Protein Sequencing Made Scalable, Accessible, and Insightful

Founded in 2013, Quantum-Si is transforming proteomics by making next-generation protein sequencing accessible through the world’s first single-molecule protein sequencing platforms, Platinum® and Platinum® Pro. By combining biology, chemistry, and AI-driven analysis, the technology enables high-resolution protein characterization to accelerate scientific discovery and drug development.

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