FluoroMax Plus

FluoroMax Plus Spincotech

Steady State and Lifetime Benchtop Spectrofluorometer

The FluoroMax® Plus represents HORIBA’s industry-leading fluorometer performance in a convenient, affordable, easy-to-use benchtop model. The FluoroMax family, with its unique all reflective optics and photon counting was the first to bring the sensitivity of a modular fluorometer to a tabletop fluorescence instrument, in the 1990’s.
It’s a compact spectrofluorometer, yet offers the ultimate sensitivity in fluorescence investigations as well as features not found in most table-top fluorescence detection systems.
The FluoroMax Plus is the latest generation of the original, high performance tabletop fluorometer, which offers extended performance with detection of emission spectra out to 1700 nm and (TCSPC) Time Correlated Single Photon Counting lifetime measurements as short as 25ps.
FluoroMax Plus spectrofluorometers are the most senstive, flexible and trusted benchotop spectrofluorometers in the world.

Features

Key Benefits
  • Water Raman signal-to-noise ratio: 10,000:1 (FSD method)
  • Photon Counting for Ultimate Sensitivity
  • Fast Scanning capability – up to 80 nm/second
  • Powerful FluorEssence™ software for Windows®
  • Method Files to recall complete experiment parameters
  • Time-correlated single-photon counting (TCSPC) accessory for lifetime determinations
  • Phosphorimeter accessory for long-lived luminescence determinations

Hardware

The Source
The xenon source that supplies prime UV performance is focused onto the entrance slit of the excitation monochromator with an elliptical mirror. Besides ensuring efficient collection, the reflective surface keeps all wavelengths focused on the slit.
As an option an additional, software-selectable, xenon flash lamp can be fitted to enable phosphorescence studies.
Optional Pulsed Laser and LED Light Sources.
The Slits

The slits themselves are bilaterally, continuously adjustable from the computer in units of bandpass or millimeters. This preserves maximum resolution and instant reproducibility.

The Excitation Monochromator

The excitation monochromator is an aspheric design which ensures that the image of the light diffracted by the grating fits through the slit. The gratings themselves are plane, blazed gratings that avoid the two major disadvantages of the more common concave holographic gratings: poor polarization performance and inadequate imaging during scans that throws away light. The unique wavelength drive scans the grating at speeds as high as 80 nm/s. The grating grooves are blazed to provide maximum light in the UV and visible region.

The Reference Detector

Before the excitation light reaches the sample, a photodiode reference detector monitors the intensity as a function of time and wavelength. The photodiode detector has a wider wavelength response than the older, traditional rhodamine-B quantum counter, and requires no maintenance.

The Sample Chamber

A spacious sample chamber is provided to allow the use of a long list of accessories for special samples, and encourages the user to utilize a variety of sample schemes.

The Emission Monochromator

All the outstanding features of the excitation monochromator are also incorporated into the emission monochromator. Gratings are blazed to provide maximum throughput in the visible region.

The Emission Detector
Emission detector electronics employ photon-counting for the ultimate in low-light-level detection. Photon-counting concentrates on signals that originate from fluorescence emission, ignoring smaller signals originating in the detector tube (PMT). The more common method of analog detection (used by lower performance fluorometers) simply adds noise and signal together, masking weak emissions.
The emission detector housing also contains an integral high-voltage supply which is factory set to provide the signal-to-noise ratio.
Computer Control

The entire control of the FluoroMax originates in your PC with our FluorEssence™ software and is transmitted through a USB link. On start-up, the system automatically calibrates and presents itself for new experiments, or stored routines instantly called from memory.

Accessories

A wide variety of accessories are applications-oriented and most are interchangeable with the FluoroMax system.

Specifications

LampVertically mounted, CW, 150 W Ozone-free xenon arc lamp
Gratings1200 groove/mm blazed at 330 nm (excitation) and 500 nm (emission), plane ruled
 Automatic self-calibration of all wavelength drives and slits
MonochromatorsAll reflective optics, Czerny-Turner spectrometers
DetectorsEmission: R928P photon counting PMT (185–850 nm) and reference photodiode for monitoring lamp output
Water Raman S/N10,000:1 (FSD method). See Signal-to-noise ratio
SlitsContinuously variable from 0 to 29 nm
Accuracy0.5 nm
Repeatability0.1 nm
Minimum step0.0525 nm
Integration time0.001 to 160 sec
SoftwareFluorEssence
Spectral Correction FactorsIncluded