Mass-directed fractionation using MS molecular weight information ensures that target compounds are collected with a high degree of certainty. Furthermore, purity confirmation is possible by subjecting the fractions to LC-MS analysis. When large numbers of fractions are obtained, optimization of the purification / fractionation conditions can be conducted with greater efficiency through high-speed analysis. The highly acclaimed Nexera Prep System offering a wide choice of separation platforms [High Precision Analytical to Semi-Preparative (up to 10 mL/min), Semi-Preparative & Recycle Preparative (up to 20 mL/min) and large scale preparative purification (up to 150 mL/min)], ably supported by highly flexible fraction collector (up to 3240 test tubes) or combined unit that performs as autosampler for sample injection and fraction collector along with LCMS-2050 single quadrupole mass spectrometer offers an excellent choice for the Mass Directed Purification.
LCMS-2050, a powerful tool for even large molecular weight compounds such as oligonucleotides, peptides, proteins, polymers apart from low-molecular weight compounds is ideally suited for mass-based purification. With its heated ion source coupled with DUIS ionization involving both ESI & APCI techniques, it provides excellent sensitivity for compounds of diverse polarities and chemistries. Thanks to the UFscanning (15,000 u/sec) and UFswitching (10 msec) it enables high speed analysis for establishing the MS-based triggers for purification with a high degree of confidence.
