Nexera XS inert Bio-inert UHPLC

The Nexera XS inert UHPLC offers the ideal solution for the separation of biomolecules using SEC, IEX, and HIC chromatography modes by combining the elevated pressure tolerance of a UHPLC system with complete inertness of the sample flow path, ensured by the absence of wetted metal surfaces and offering ultra-high resistance to corrosion, caused by mobile phases with high salt concentrations and extreme pH values.  The same outstanding instrument performance capabilities offered by N-Series systems are also available in Nexera XS inert systems. For more advanced analysis, Nexera XS inert can be used with Analytical Intelligence technology for automatic analysis, analytical method development using method scouting, quality control on biologics etc.

Full-range of Bio-inert UHPLC capabilities

For the quantitative analysis of biomolecules, particularly when high sensitivity is required, the adsorption of the target compound to a metal surface can result in a dramatic decrease in sensitivity. The Nexera XS inert system prevents peak tailing and sensitivity loss by eliminating adsorption of proteins, nucleic acids, and other components to metal surfaces. As a result, reliable and accurate quantitation is possible, even for low-concentration samples.

The Nexera XS inert system is equipped with unique technology that ensures the complete inertness of the sample flow path providing excellent peak shape and unsurpassed chromatographic separation.

Adsorption of target compounds can be especially detrimental in the low-concentration region, and can worsen calibration curve linearity. The Nexera XS inert achieves a wider dynamic range by strongly increasing the quantitative accuracy and detection of low-concentration samples.

Flow Channel Switching Valves for Metal-absorbing Compounds

All valves used in Nexera XS inert systems are designed to inhibit metal adsorption. That ensures worry-free switching between multiple columns for method development or even for trap-and-elute analysis without being concerned about adsorption.

Mobile Phase pH Monitor (pHM-40) (optional)

In the case of Ion-exchange chromatography or size-exclusion chromatography used for the analysis of proteins or other biomolecules, the mobile phase pH can strongly affect chromatographic separation. The pH monitor pHM-40 continuously monitors the pH of mobile phases to identify any changes in mobile phase pH in real time.

Analytical Columns for Target Compounds

Analyzing biomolecules, such as proteins and nucleic acids, requires using columns intended for a variety of separation modes. In particular, samples sensitive to metal ions require a column that is optimized to reduce adsorption.  Shimadzu offers columns optimized for a wide variety of molecules.

Oligonucleotide mixture containing impurities  

mAb Aggregate analysis