The NucleoCounter® NC-250™ is an automated cell analyzer, which can characterize cell properties using fluorescence imaging. The NC-250™ offers a wide range of optimized protocols to meet the needs of all mammalian cell types grown under different conditions.
Through unique assays, cell count can be performed even on cells growing on microcarriers and in sphere cultures. This benchtop image cytometer ensures high-precision and does not require calibration.
With a combination of simplicity and innovation, counting and viability determination of mammalian cells has never been easier. Simply mix a small volume of cell suspension with fluorescent dyes, load, and run. Within three minutes you will have eight highly precise viability and cell count analyses at hand.
For cells with low concentration, or to achieve even higher precision, users can take advantage of the NC-Slide A2™ with a 30 µl volume capacity.
The NucleoCounter® NC-250™ automated cell analyzer performs high-speed cell count and viability determination and offers a unique 5-minute Cell Cycle Assay and a 1-minute Apoptosis Assay for up to eight samples at the time.
The intuitive software has unlimited licenses and produces on-screen data analysis and automated PDF reports.
| Application type(s) | Cell count & viability, Cell cycle, Vitality |
| Cell type(s) | Mammalian |
| Software | NucleoView™ |
| Consumable(s) | NC-Slide A2™ and NC-Slide A8™ |
| Capacity (Analysis time) | Variable, depending on consumable and assay type |
| Loading volume | 30 μl (NC-Slide A2™), 10 μl (NC-Slide A8™) |
| Analysis volume | 0.8 – 16 μl sample (NC-Slide A2™, NC-Slide A8™) |
| Optimal range | 5×104 to 5×106 cells/ml |
| Staining dye(s) | Acridine orange (AO), DAPI, VitaBright-48™, Propidium iodide (PI), User-defined |
| Excitation | Two LED light sources with peaks at 365 nm and 500 nm + one dark field white LED |
| Emission (nm) | A single dual-band emission filter: 410 – 450 nm and 520 – 595 nm |
| Optics | Lens with ×1.75 magnification, 1/2.5” CMOS camera |
| Data output | Images, tables, histograms, scatter plots |
| Data export formats | .cm, .csv, .pdf, .cmpp, .bmp, .tiff, .acs, .fcs |
| Product Number | 970-0251 |
| Size | 19 × 29 × 32 cm (8 × 12 × 13”) (W × H × D) |
| Weight | 9 kg (20 lbs) |
| Supply voltage | 24VDC (100–240 V ~ 50–60 Hz) |
| Product type | Product No. | Product name |
| Sampling devices | 942-0001 | NC-Slide A2™ |
| 942-0003 | NC-Slide A8™ | |
| Software | NucleoView™ | |
| Solutions | 910-0003 | Reagent A100 Lysis Buffer, 500 ml |
| 910-0002 | Reagent B Stabilizing Buffer, 500 ml | |
| 910-3002 | Solution 2 Propidium Iodide Staining Reagent, 25 ml | |
| 910-3003 | Solution 3 DAPI Staining Reagent, 25 ml | |
| 910-3006 | Solution 6 VB-48™ PI Staining Reagent, 1 ml | |
| 910-3010 | Solution 10 Lysis Buffer, 100 ml | |
| 910-3011 | Solution 11 Stabilization Buffer, 100 ml | |
| 910-3012 | Solution 12 DAPI Staining Reagent, 1 ml | |
| 910-3017 | Solution 17 Blood Lysis Buffer, 25 ml | |
| 910-3018 | Solution 18 AO•DAPI Staining Reagent, 1 ml | |
| Accessories | 930-0005 | Power Supply NucleoCounter® NC-200™, NC-250™, SCC-400™ |
| 911-0013 | NucleoCounter® Cleaning Kit |