The NucleoCounter® NC-200™ automated cell counter is a high-precision instrument utilizing state-of-the-art image cytometry. It counts suspension cells, aggregated cells, CAR-T cells, stem cells and cells on microcarriers.
The NucleoCounter® NC-200™ automated cell counter comes with optimized analysis settings for cell count and viability measurements. Along with its user-adaptable protocols and specialized assays, the NC-200™ is an all-in-one solution for cell counting and cell viability determination.
Use the Via1-Cassette™ with this cell counter for automated staining and sample handling. The disposable cassette is volume-calibrated to ensure high-precision counts and cell reproducibility.
| Application type(s) | Cell count & viability for mammalian cells |
| Cell type(s) | Mammalian cells, including cell lines, leukopaks, PBMCs, whole blood, MSCs, T-cells, aggregated cells, & microcarriers |
| Software | NucleoView™ |
| Consumable(s) | Via1-Cassette™ |
| Capacity (Analysis time) | 50 seconds (one step), 120 seconds (two steps) |
| Loading volume | 60 μl |
| Analysis volume | 1.4 μl |
| Optimal range | 5×104 to 5×106 cells/ml |
| Staining dye(s) | Acridine orange (AO), DAPI |
| Excitation | 2 LED light sources peaking at 365 nm and 505 nm, dark field light |
| Emission (nm) | A single dual-band emission filter: 410 – 460 nm and 540 – 650 nm |
| Optics | Lens with x1.3 magnification, 1/2.” CMOS camera |
| Data output | Images, tables, histograms, scatter plots |
| Data export formats | .cm, .csv, .pdf, .cmpp, .bmp, .tiff |
| Product Number | 900-0200 |
| Size | 40 × 23 × 25 cm (W × H × D) |
| Weight | 4.5 kg (9.9 lb) |
| Supply voltage | 24VDC (100-240V ~ 50-60Hz) |
Sampling Devices, Reagents & Test Kits for the NucleoCounter® NC-200™ Automated Cell Counter
| Product type | Product No. | Product name |
| Sampling Devices | 941-0012 | Via1-Cassette™ |
| 941-0024 | Via2-Cassette™ | |
| Service Plan | 980-5200 | Service Plan for NucleoCounter® NC-200™ |
| Software | 950-0201 | NucleoView™ |
| Reagents | 910-0003 | Reagent A100 Lysis Buffer, 500 ml |
| 910-0002 | Reagent B Stabilizing Buffer, 500 ml | |
| 910-3010 | Solution 10 Lysis Buffer, 100 ml | |
| 910-3017 | Solution 17 Blood Lysis Buffer, 25 ml | |
| Accessories | 930-0005 | Power Supply NucleoCounter® NC-200™, NC-250™, SCC-400™ |
| 929-0012 | Laptop Stand II | |
| 929-0010 | Short LID Yellow |