Read More What Does High-Quality Spatial Genomics Data Look Like? Ebook MERSCOPE™: Delivering high-sensitivity, high-specificity spatial genomics data to reveal biology with true single-cell precision. Spatial Transcriptomics Plunge into the inner life of cells with Vizgen’s integrated spatial multiomics platforms. Contact Us arrow_forward arrow_back Vizgen Bg Image Slider Revolution Spincotech Vizgen white Logo Spincotech

How MERFISH 2.0 Works

Multiplexed Error-Robust Fluorescence in situ Hybridization

Reveal deeper layers of biological complexity with MERFISH 2.0, a single-molecule FISH-based imaging technology for spatially resolved transcriptomics, where optimized chemistry drives sharper resolution for mapping transcripts and cell types with greater precision.
MERFISH achieves unmatched multiplexing and subcellular resolution by assigning each RNA transcript a unique binary barcode decoded through iterative imaging rounds for accurate, reproducible results. Optimized probe chemistry and enhanced readout design boost sensitivity and specificity, even in degraded or archival FFPE samples, expanding transcript detection across diverse tissue types.

By localizing transcripts with nanometer precision, MERFISH 2.0 reveals the spatial organization of genes, cell states, and interactions, creating molecular maps that link expression to function.

MERFISH 2.0 is Built Upon MERFISH Chemistry

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Map Challenging Tissues

Acquire high-quality, reproducible spatial data from even the most difficult samples, including archival FFPE and fresh-frozen tissues.

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High Dynamic Range Transcript Capture

Improved sensitivity detects more RNA molecules with uncompromised specificity and subcellular resolution, revealing subtle expression gradients.

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More Cells, More Insights

Generate richer datasets with more cells passing quality filters, enabling higher transcript detection and deeper biological context per sample.

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Identify Cell Types and States

Distinguish rare and abundant cell populations across complex tissues to illuminate the mechanisms that drive health and disease.

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Uncover More Spatial Interactions

Explore molecular and cellular crosstalk within intact tissue architecture to inform disease models and therapeutic discovery.

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Proven, Scalable MERFISH Chemistry

Built on decades of MERFISH spatially resolved transcriptomics innovation, MERFISH 2.0 delivers scalable, high-confidence results.

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The Vizgen advantage

How Our Technologies Power Applications

MERFISH 2.0 on the MERSCOPE Ultra platform resolves tens of thousands of RNA targets per sample at true single-cell resolution, revealing cell states and spatial organization.

InSituPlex multiplex immunofluorescence quantifies protein markers on the same types of specimens, including FFPE.

Together, these technologies deliver true multiomics that turn static sections into actionable spatial biology for neuroscience, tumor profiling, immune research, and many more fields of research.

Applications

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Neuroscience

Detect brain cell types and circuits in intact tissue with MERFISH spatial transcriptomics or multiplex proteomics.

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Oncology

Profile the tumor microenvironment: map up to 1,000 genes at single-cell scale and quantify checkpoint proteins with multiplex IF to inform therapy response.

Immunology

Map immune niches with spatial transcriptomics or multiplex proteomics to profile cell states, interactions, and checkpoint/activation markers in context.

Our Expertise

Vizgen enables high-resolution spatial transcriptomics using MERFISH technology to map thousands of RNA molecules within intact tissues at single-cell resolution, delivering deeper insight into tissue architecture and cellular function.

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MERSCOPE Installations Globally

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MERFISH Peer-reviewed & pre-print publications