Figure1: The amount of light scattered by a particle scales linearly with the particle’s volume and refractive index. As the optical properties and density of biomolecules vary only by just a few percent, the scattering signal is directly proportional to the molecule’s mass – making it possible to weigh single molecules with light (Figure). The correlation of scattering signal with mass holds true for a variety of biomolecules (e.g. glycoproteins, nucleic acids and / or lipids), making mass photometry a universal analysis tool for biomolecules in solution.
With a wide dynamic range, mass photometry can be used to assess:
Figure 2: The principle of mass photometry. The light scattered by a molecule that has landed on a measurement surface interferes with light reflected by that surface. The interference signal scales linearly with mass.
Precisely characterize AAV preparations by accurately determining empty versus full capsid ratios, aggregation states, and overall integrity. Crucial for optimizing production and ensuring the safety and efficacy of gene therapies.
Gain direct, label-free insights into AdV quality by assessing particle concentration, aggregation, and purity. This enables better control over manufacturing processes for gene delivery vectors and oncolytic agents.
Accurately detect and quantify antibody aggregates in solution – faster and with far less sample than SEC and AUC. Essential for optimizing formulation conditions, improving stability, and ensuring safe, effective biotherapeutics.
Comprehensively profile bispecific and multi-specific antibody-antigen interactions, including stoichiometry. Ideal for new modalities, complements SPR and BLI, aiding experimental design and the interpretation of data on multi-component systems.
Analyze membrane proteins in native-like environments, determining oligomeric states, complex formation, and sample purity. Critical for advancing structural studies, functional assays, and therapeutic development.
Characterize mRNA integrity and its formulation in lipid nanoparticles (LNPs). Assess size distribution, encapsulation efficiency, and stability of mRNA-LNP complexes, vital for developing and quality controlling mRNA-based vaccines and therapies.
Directly measure complex formation, stoichiometry, and binding strength to reveal how proteins interact in solution. Vital for understanding biological mechanisms, validating targets, and accelerating drug discovery.
Our advanced single‑particle analysis reveals population heterogeneity and sample purity. It delivers precise, multiparametric data by simultaneously measuring particle size and mass-related contrast.
At the heart of Refeyn’s innovation lies a commitment to providing truly insightful biomolecular data without the traditional trade-offs. Our mass photometry platform is engineered to deliver clear, actionable results quickly and simply, fundamentally changing how you approach characterization.
Our new MyMass mass photometer delivers fast, accurate assessment of purity and homogeneity so you can move forward with cryo-EM, native MS, SPR or BLI with confidence.
