LabSolutions Insight Biologics is software for analyzing the characteristics of oligonucleotides using single quadrupole (SQ) mass spectrometers, quadrupole time-of-flight (QTOF) mass spectrometers, or MALDI mass spectrometers. Analyzing the characteristics of oligonucleotides involves the identification of impurities and sequence confirmation with modification positions estimated. Performing these analyses using a complicated mass spectrum requires a great deal of time. Additionally, using multiple software programs for the measurements and data analysis complicates the operations and puts considerable strain on the operator.<br>LabSolutions Insight Biologics has been designed to resolve these issues.
With LabSolutions Insight Biologics, it is easy to configure the primary component sequence and data analysis parameters. Then using the sequence entered, the program can comprehensively identify chain length differences, nucleotide gaps, modifications, ion adducts, and other impurities. The molecular weights of the primary components and impurities are estimated with the MS1 spectrum, and the sequence is estimated from the fragment spectra of the impurities. Also, the sequence validity can be confirmed by visually checking the fragment coverage.
LabSolutions Insight Biologics is all-in-one LabSolutions series software. After measurements with LabSolutions, the data analysis is implemented automatically with the specified parameters, so when the measurements are started, everything up to the display of the analysis results is performed seamlessly.
In addition to QTOF, LabSolutions Insight Biologics can analyze SQ and MALDI data. When QTOF is used, high-accuracy results are obtained, while SQ and MALDI can be used for applications where convenient measurement is prioritized. In process management, everything from the establishment of efficient processes to productivity improvements can be implemented. In addition, it can be used in research and development in a variety of fields.
The data analysis parameters can be conveniently deployed even in workflows with different instruments, so there is no need to remember new operations.
In the window for setting the oligonucleotide sequences,the structural formula of the sequence that was entered is displayed in real time, enabling quick and easy verification of the information. Also, the nucleobases used in the sequence, as well as the backbone linker, ribose, and base modifications, can be added and edited.
The software includes a coverage display which indicates fragment spectral assignments. The coverage display switches to match the items to be checked. Reports can also be output.
Using the peak information from the MS spectrum simultaneously with the integrated LC chromatogram peaks enables purity calculations even for components that are insufficiently separated in the LC chromatogram.
Using the peak information from the MS spectrum simultaneously with the integrated LC chromatogram peaks enables purity calculations even for components that are insufficiently separated in the LC chromatogram.
The software includes a coverage display, which indicates fragment spectral assignments. The coverage display switches to match the items to be checked. Reports can also be output.
Given the complex nature of synthesizing oligonucleotide therapeutics Insight Biologics is designed to positively identify each product related component with high confidence.
By supporting both positive and negative ionization modes it enables data analysis using ion pair reagents in negative ion and HILIC separations in positive ion (for example analyzing morpholino nucleic acid in positiveion).
