With a high-density SMRT Cell, up to 4 SMRT Cells per run, and 24-hour run times3, the Revio system with SPRQ chemistry delivers up to 480 Gb2,5 of HiFi reads per day, equivalent to 2,500 human whole genomes4 per year.
SPRQ chemistry on the Revio system unlocks the ability to sequence more sample types than ever before, using just 500 ng of native DNA – without sacrificing the high throughput or exceptional quality you rely on.
SPRQ chemistry, paired with Google Health DeepConsensus algorithms, delivers exceptional read accuracy plus confident 5mC and 6mA methylation detection in every run. Optimized file formats reduce data storage needs through quality value binning and smart read ordering, streamlining data handling and maximizing efficiency.
Take a 3D tour of the Revio system to see key features, setup, workflows and more.
With SPRQ chemistry, the Revio system helps you achieve more with less DNA, increasing efficiency, maximizing output, and reducing costs – empowering you to make more discoveries faster and affordably.
Resolve chromatin architecture with Fiber-seq powered by on-instrument 5mC and 6mA calling, now with an increased 5mC CpG accuracy for even greater precision.
The Revio system with SPRQ chemistry produces exceptional read length, quality, and variant calling performance that have made HiFi sequencing so celebrated.
The complete, gapless human genome marks a new era of genomics where no region of the genome is beyond your reach. In 2021, PacBio HiFi technology was used by the Telomere-to- Telomere (T2T) Consortium to fill in those gaps.
HiFi reads are tens of kilobases long, providing the ability to resolve large variants and map to difficult regions of the genome.
The Revio system provides reliable answers through exceptional accuracy, with 90% of bases Q30+ and median read accuracy ≥Q30.
HiFi sequencing with SPRQ chemistry on the Revio system delivers high accuracy variant detection, resulting in fewer sequencing errors relative to other technologies at lower coverage1.
The Revio system simplifies the HiFi sequencing experience with fewer consumables optimized for easy tracking and minimal waste. With workflow improvements, loading consumables takes under a minute at the instrument.
One tray contains 4 high-density SMRT Cells.
The sequencing plate provides reagents for sequencing up to 4 Revio SMRT Cells.
The Revio system gives you a vastly simplified experience with consumables and software designed for flexibility and convenience. Whether you’re a genome center or a small lab, you can enjoy:
Library prep for the Revio system supports manual and automated prep.
Sequencing plates are linked automatically to run designs through an NFC tag, enabling runs to be set up in less than one minute on instrument.
The Revio work deck is isolated from the four sequencing stages, leaving the system available up to 20 hours per day for loading consumables for subsequent runs.
