Advanced scientific discoveries require sequencing data that is both accurate and complete. PacBio sequencing technology has evolved to a different type of long read, known as highly accurate long reads, or HiFi reads.
PacBio is the only sequencing technology to offer HiFi reads that provide accuracy of 99.9%, on par with short reads and Sanger sequencing. With HiFi reads you no longer compromise long read lengths for high accuracy sequencing to address your toughest biological questions.
Up to 25 kb in length
Sequencing free of systematic errors
No bias even for high GC regions
Direct methylation without special library prep
Discover how HiFi sequencing is transforming research directly from the scientists who use the technology. It’s more than just about long read lengths and high accuracy—it’s about making a real impact in the field.
Access high accuracy for all variant types — SNVs, indels, structural variants, tandem repeat expansions, and methylation — including in challenging regions.
Accurately construct the full sequence of chromosomes, including telomeres and centromeres.
Focus the power of HiFi variant calling by enriching for regions of interest using hybrid capture or PCR amplification.
Catalog RNA isoforms at single-cell resolution, moving beyond gene counting to catalog full length transcripts.
Achieve the highest consensus accuracy and uniform coverage for reference quality genomes in humans, plants, animals, and microbes.
Explore how epigenetic changes affect gene expression, host-pathogen interactions, environmental response, and more.
Accurately detect and uncover all variant types even in hard-to-reach regions of the genome.
Discover new genes, transcripts, and alternative splicing events with a complete view of transcript isoform diversity.
The Revio system runs on SPRQ chemistry and helps you achieve more with less DNA, empowering you to make more discoveries faster and more affordably. New SPRQ-Nx extends SPRQ chemistry with improved consistency, a new 5hmC methylation caller, and multi-use SMRT Cells, enabling a richer and cost-effective HiFi workflow. Built for projects that scale, it maintains the gold-standard HiFi quality you rely on, from population studies to routine panels.
Pacific Biosciences advances genomics through long-read HiFi sequencing technology, delivering highly accurate and contiguous reads for comprehensive analysis of complex genomic variations, structural variants, and repeat regions beyond conventional sequencing approaches.
