Shimadzu’s Solution for accelerated analytical method development in HPLC is ably supported by ‘Automated method scouting and LabSolutions™ MD, the software that takes full advantage of the Analytical Quality by Design (AQbD) approach’. LabSolutions MD efficiently develops highly reliable analysis methods by configuring mobile phases, columns, and other parameters using an analysis function that automatically generates analysis schedules with the experimental design method and a data analysis function that plots a design space and predicted chromatogram.
All steps involved in the screening, optimization, and validation phases of the method development workflow can be completed using LabSolutions MD. These include analyzing samples using experimental design, building a design space using the analytical results, and evaluating robustness after deciding the optimal analytical conditions.
Method Scouting Solution dedicated software automates the switching of column and mobile phase combinations, leading to efficient and reliable method development.
In manual method scouting, a method must be created every time the column or mobile phase is switched. For e.g., 100 combinations of analytical conditions lead to 100 methods – not only highly labour-intensive but also increasing the risk of operational errors. Method Scouting System is intuitive to operate and can automate these changes in analytical conditions, allowing resources to be used more efficiently.
The mobile phase blending function allows quick adjustments to the salt concentration, pH, and organic solvent ratio on-line using only a few pre-prepared solvents, and quickly search for suitable conditions. This significantly reduces the time required to prepare and evaluate mobile phases. The system can also perform different types of mobile phase blending, two for water-based and two for organic solvents, using a low-pressure gradient.
The data can be evaluated quantitatively with a multi-data report. Shimadzu’s evaluation method, based on the degree of separation and the number of peaks detected, can quantitatively analyze chromatograms to derive the optimum method. Registered templates provided in Method Scouting Solution and the report will be produced as soon as the analysis is completed, so the optimum conditions can be checked right away.
