MSD - Meso Scale Discovery - Spincotech

Design of biomarker multiplex assays to support research on CAR-T cell efficacy, safety, persistence and exhaustion

Tech Note

Multiplex protein biomarker assays enable monitoring of key CAR-T therapy attributes such as efficacy, safety, persistence, and exhaustion, supporting insights into treatment dynamics, while CAR-T therapies have transformed cancer care with strong responses but can be associated with adverse events like CRS and ICANS.

ECL Multiplex Assays

MSD's electrochemiluminescence (ECL) detection technology uses SULFO-TAG™ labels, which emit light upon electrochemical stimulation initiated at the electrode surfaces of MULTI-ARRAY and MULTI-SPOT® microplates.

Electrochemiluminescence

Electrochemiluminescent labels generate light when stimulated by electricity in the appropriate chemical environment. This reaction is incorporated into our immunoassays to provide the light signal used to measure important proteins and other biomedical molecules.

How does electrochemiluminescence work?

High binding carbon electrodes in the bottom of MULTI-ARRAY and MULTI-SPOT microplates allow for easy attachment of biological reagents (10X greater binding capacity than polystyrene).
MSD assays use electrochemiluminescent labels that are conjugated to detection antibodies. The labels are called SULFO-TAG, and allow for ultra-sensitive detection.
Electricity is applied to the plate electrodes by an MSD instrument leading to light emission by SULFO-TAG labels. Light intensity is then measured to quantify analytes in the sample.

Advantages of Electrochemiluminescence:

High sensitivity: Multiple excitation cycles can amplify signals to enhance light levels.
Broad dynamic range: The wide dynamic range of our detection systems means high and low expression levels can be measured without multiple sample dilutions.
Low background: The stimulation method (electricity) is decoupled from the signal (light) allowing only labels near the electrode surface to be detected.
Easy to use: Immunoassay method similar to conventional ELISA, but quicker.
Great flexibility: Labels are stable, non-radioactive, and conveniently conjugated to biological molecules.
Unsurpassed performance and quality: Electrochemiluminescence is a highly successful detection system that achieves clinical quality data in a variety of sample types, including cell supernatant, serum, plasma, and whole blood.

Applications

List Of Applications

Personalized Medicine
Companion Diagnostics
Vaccine Studies
Biomarker Discovery
Screening and Lead Optimization
Assay Development
Immunogenicity
Preclinical and Clinical Studies

our expertise

A Solution for Every Lab

MSD’s industry-leading immunoassay analyzers deliver 5 logs of dynamic range, attomolar sensitivity, multiplexing capability, and access to a menu of over 600 assays.

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