Protein Barcoding with Next-Gen Protein Sequencing™
Next-Generation Protein Sequencing™ (NGPS™) offers single-molecule sequencing of multiplexed protein barcodes with quantitative results.
Drug Delivery
Advance Drug Delivery and Protein Analysis with Precision Barcoding
Evaluate and optimize drug delivery vehicles like LNPs or AAVs with protein barcodes
Characterize multiplexed drug delivery and translation efficiency of mRNA therapeutics with lipid nanoparticles (LNPs). Multiplexed analysis of target therapeutics and drug delivery systems accelerates screening cycles, reduces resources of in vivo and in vitro models, and saves time.
Barcode mix encoded as mRNA and packaged into LNPs
Inject into mouse model; harvest target tissues
Enrich for target protein; functionalize, cleave, and sequence barcodes
Protein Engineering
Efficiently analyze protein characteristics in multiplex with protein barcodes
Screen Multiplexed Protein Characteristics in Protein and Enzyme
Multiplexed analysis of engineered proteins with peptide barcodes offers single-molecule precision and relative quantitation of expression.
How it Works
Protein barcoding provides users with a robust method to screen multiple protein variants in a single pooled reaction. Individual proteins are tagged with short, unique peptide barcodes that identify each variant. With the barcoding kit, screened proteins are prepared and barcodes are released for sequencing on Platinum® Pro.
Prepare Peptide Barcodes For Single-Molecule Sequencing
Sequence Peptide Barcodes With Single-molecule Precision
Analyze Multiplexed Barcodes With Relative Quantitation
Barcoding Kit
Provides methods for expression of unique peptide barcodes in proteins of interest for downstream sequencing and analysis on Platinum Pro.
The Barcoding Kit contains reagents to prepare and immobilize barcode-tagged peptides for sequencing. Eight unique peptide barcodes are carefully designed for optimal sequencing performance with the Sequencing Kit.
Sequencing Kit
Contains ready-to-sequence chips, recognizers, and aminopeptidases.
When used with the Barcoding Kit, eight peptide barcodes can be uniquely identifed in a multiplexed sequencing run, providing relative quantitation with a tenfold dynamic range.
