Shim-pack Scepter LC Columns

Shim-pack Scepter LC columns, the next generation organic silica hybrid based columns from Shimadzu, offer excellent stability and performance over a wide range of LC conditions. With different chemistry characteristics, Shim-pack Scepter columns are effective for method development/scouting with suitability for use in a wide variety of applications. With different particle sizes (1.9 μm, 3 μm, 5 μm) and different column dimensions, Shim-pack Scepter LC columns are fully scalable between UHPLC, HPLC and preparative LC making method transfer seamless between different laboratory instrumentation.

Shim-pack Scepter Chemistries

Method Scouting

With excellent stability under a wide range of LC conditions, Shim-pack Scepter LC columns are effective for method scouting combining mobile phase pH and organic modifier.

Comparison of Chromatograms using Gradient condition with Acetonitrile

C18 HD-C18 C8 Phenyl PFPP
pH 2.9
pH 6.1
pH 9.9 Outside of recommended pH


  • Saccharin (pKa = 2.2)
  • Dextromethorphan (pKa = 8.3)
  • Amitriptyline (pKa = 9.4)
  • N-Butyl paraben (pKa = 8.3)
  • Ibupurofen (pKa = 4.4)