Shim-pack Scepter LC Columns
Shim-pack Scepter LC columns, the next generation organic silica hybrid based columns from Shimadzu, offer excellent stability and performance over a wide range of LC conditions. With different chemistry characteristics, Shim-pack Scepter columns are effective for method development/scouting with suitability for use in a wide variety of applications. With different particle sizes (1.9 μm, 3 μm, 5 μm) and different column dimensions, Shim-pack Scepter LC columns are fully scalable between UHPLC, HPLC and preparative LC making method transfer seamless between different laboratory instrumentation.
Shim-pack Scepter Chemistries
Method Scouting
With excellent stability under a wide range of LC conditions, Shim-pack Scepter LC columns are effective for method scouting combining mobile phase pH and organic modifier.
Comparison of Chromatograms using Gradient condition with Acetonitrile
| C18 | HD-C18 | C8 | Phenyl | PFPP | |
|---|---|---|---|---|---|
| pH 2.9 |  |
 |
 |
 |
 |
| pH 6.1 |  |
 |
 |
 |
 |
| pH 9.9 |  |
 |
 |
 |
Outside of recommended pH |
Analytes
- Saccharin (pKa = 2.2)
- Dextromethorphan (pKa = 8.3)
- Amitriptyline (pKa = 9.4)
- N-Butyl paraben (pKa = 8.3)
- Ibupurofen (pKa = 4.4)
