Nexera UC Prep

Nexera UC Prep Preparative supercritical fluid chromatography system

The Nexera™ UC Prep  preparative supercritical fluid chromatograph offers both the high basic performance and state-of-the-art preparative SFC technologies. It resolves a number of issues in preparative tasks, reducing labor and improving efficiency while fitting into pre-existing workflows. Not only does the Nexera UC Prep achieve superior recovery rates for purification, it provides flexible system configurations in a compact design, requiring low installation space and allows maximization of laboratory resources.  Target compounds are recovered in high concentrations in an organic solvent, which saves time not only during analysis, but also during post-run processing after preparative tasks are complete.  Fractionation output is maximized with high recovery rates coupled with ability to carry out continuous preparative work, shortening overall process times.

Unique LotusStream™ separator technology achieves higher recovery rates

Unique gas-liquid separation technology is used to minimize the potential for low sample recovery due to eluate scattering during carbon dioxide vaporization, achieving high recovery rates even for volatile compounds


Simple operation fits seamlessly into preparative workflow

The dedicated software with intuitive preparative settings ensures peaks can be separated reliably by users of all experience level.  The parameter settings in Prep Solution have been kept as concise and intuitive as possible, so that all users can operate the system with minimal training. This also avoids the risk of wasting samples through human error.

A trial analysis, or scouting run, prior to fractionation confirms component peak shapes and retention times. Analysis can be started by simply inputting basic parameters in the three onscreen tabs.

The chromatogram obtained from the single analysis can be displayed in the simulation window, so that the collection start and stop times for each fraction can be selected with just a few mouse clicks. These settings can be applied to methods automatically.

Samples are fractionated based on the user-selected parameters. The fraction range is displayed on the chromatogram, which can be checked in real time.

Parameter settings for fractionation and injection can be adjusted during stacked injection (“on-the-fly” function).

Compact, benchtop design

This space-saving benchtop model includes a carbon dioxide pump that does not require an external chiller (cooling system for heat generated when pumping CO2 at high ow rates). In addition, one unit can handle a wide range of row rates, lowering installation costs.

Add fractionation capabilities to the analytical SFC system

By adding a fraction collector to the analytical scale SFC, small volume fractionation is also possible. A complete worklow can be carried out seamlessly on one system, from method development to fractionation.   Variety of options as listed below, support a wide application range.

  Stacked Fraction System Multi-Fraction System Analytical Fraction System
Flow rate range 10.0 – 150.0 mL/min 0.0001 – 5.0 mL/min
Supported columns I.D.: 10 – 30 mm
Length: up to 250 mm
I.D.: 2.0 – 4.6 mm
Length: up to 250 mm
Injection unit No. of samples processed: 1

Max. injection volume: 2 mL, (optional: 20 mL)


No. of samples processed:
252 (with 1 mL sample vial plate)
162 (1.5 mL sample vial plate)
84 (4 mL sample vial plate)
36 (10 mL sample vial plate)
72 (microtube plate)
288 (96-well microplate)
1,152 (384-well microplate)Max. injection volume: 500 μL (optional: 2 mL) 
No. of samples processed:
175 (with 1-mL sample vial plate)
105 (1.5-mL sample vial plate)
50 (4-mL sample vial plate)
192 (96-well microplate)
768 (384-well microplate)
192 (96-well deep-well plate)
768 (384-well deep-well plate)Max. injection volume: 5 μL   (optional: 20 μL)
Collection Supported containers: Bottles (screw-top: GL45)
Quantity: Five 100 mL to 2 L
Collection method: Valve switching
(10 collection + 1 waste or 5 collection + 5 waste)
Supported containers: Test tubes
Quantity: 540 10-mL, 20 250-mL, or 12 500-mL test tubes
Test tube I.D.: From 10 mm
Collection method: Open-bed with X-Y arm
Volume recovered per analysis
Up to 1 g
(for 30 mm I.D. column)
Up to 20 mg
(for 4.6 mm I.D. column)
Sample temperature
control range
No temperature control 4 – 45°C 4 – 40°C
Support functions Dedicated preparative software,
stacked injection,
parameter changing during analysis
Dedicated preparative software,
parameter changing during analysis