Shimadzu Voglibose Analysis System comprises of the most popular N-Series HPLC modules coupled to a Post-column derivatization set up and the world’s highest sensitivity RF-20Axs Fluorescence detector. The system is the industry’s benchmark and provides total compliance to the regulated methods for the analysis of voglibose, an anti-diabetes drug that inhibits the activity of α-glucosidase.
Since voglibose absorbs in the low-wavelength region, it cannot be directly detected with a UV-Vis detector with high sensitivity. Therefore a post-column derivatization method with taurine/periodic acid reagent coupled with fluorescence detection, which is effective for detecting sugar alcohols and non-reducing sugars, is commonly used.
Voglibose after separation in an amino column (Shim-pack) is allowed to react with a solution of (taurine/sodium periodate) at 100 Deg C in the Post-column system and detection is done using the fluorescence detector maintained at 15 Deg C. As the RF-20Axs is equipped with a cell temperature control which can work at 15 Deg, C, the system does not need any external temperature-controlled cooling bath. Further, the system provides improved accuracy and reduces fluorescence quenching at elevated temperatures. The system provides excellent linearity at concentrations of 2-250 µg/L and meets the system suitability criteria as specified in Japanese Pharmacopoeia with an R2 value greater than 0.9999.