Nexera Prep LC is a completely scalable solution that addresses most of the current demands on isolation of target substances in pharma, natural products, proteomics or any other applications. Users can select a pump, auto sampler, and detector to build a system according to their needs. The different models of mobile phase delivery pump cover from analytical to semi-prep to full prep scale purification with the standard configuration offered with UV-VIS detection. A host of new innovations introduced in the Nexera Prep help overcome many challenges during the isolation of trace impurities in presence of large concentrations of active substances, overlap of similar compounds etc,. Systems can be configured either with FRC-40 Fraction Collector or LH-40 Liquid Handler, depending on injection volume and/or whether reinjection of the fraction is required.
Nexera Prep
Wide Choice of Preparative Options
Streamline and Simplify Conditions for Preparative Work
Nexera Prep
Using the Nexera™ Prep system saves on labor when scaling up from the development of analytical conditions to the conditions for preparative work.
Isolate Target Components at High Purity Levels & High Concentrations
The Shimadzu UFPLC, Ultra Fast Preparative and Purification Liquid Chromatograph significantly reduces the cost and labor involved in preparative purification. Additionally, the system not only performs purification of target components, but can also recover impurities with high yield enabling direct impurity analysis.
UFPLC: Ultra Fast Preparative and Purification Liquid Chromatography System
Seamlessly isolate Non-UV Absorptive Components
Preparative LCMS System
With LH-40 and FRC-40 able to perform signal-based logic and collection on up to four signal channels, not having a chromophore is not a limitation. Nexera Prep can use LCMS, RID, and ELSD to detect and/or identify targets for purification.
High Efficiency Separation via Preparative Recycling
By repeatedly cycling the sample through the column, the target component can be resolved and recovered from coeluting species or impurities without the need for longer or multiple columns.
Excellent System Expandability
LH-40 Liquid Handler and the FRC-40 Fraction Collector
LH-40 Liquid Handler and FRC-40 Fraction Collector support Nexera Prep’s excellent scalability and the column hub that can store multiple columns and valves. Both LH-40 and FRC-40 can connect up to six units to enable fractionation into up to 3240 pieces of 10 dia. test tubes. The LH-40 can be used not only as a fraction collector, but also as an autosampler with the liquid surface detection function (option). It also enables seamless purity checks through re-injection of fractions after fractionation in order to provide even more efficient preparative purification work.
Using the fractionation simulation function of LabSolutions software the time required for condition investigation can be reduced to approximately 1/4. The system also supports various racks (custom racks) on which test tubes are stored according to the recovered quantity. In addition, the system is equipped with a multi-channel function to perform preparative purification on the target compound at a high purity using detection signals from up to four detector channels, a function to recognize noises and peaks to selectively detect and fractionate target peaks (patent applied). Even if a problem occurs during preparative work, the sample remaining in the system can be recovered. By following the rescue instructions, the precious sample is recovered into the specified container rather than being discarded.
Fraction Purity Checks (LH-40)
Purification Solution™ Simplifies Settings Related to Preparative Purification
The special Purification Solution software is equipped with peak tracking functions that enable the target peaks and fractionate and be checked at a glance.
Various Fractionation Modes
Automatic Removal of Non-volatile Salts
In the conventional preparative LC, salts derived from the mobile phase are included in the recovered product. With Nexera UFPLC, salts derived from the mobile phase can be removed on the trap column. In the picture below, Ibuprofen was prepared using a solvent containing ammonium chloroacetate, a non-volatile salt. With conventional preparative LC, ammonium chloroacetate precipitated at the same time during evaporation. However, ibuprofen was recovered as a single component with Nexera UFPLC due to the use of a trap column.
Counter Ions Removal
In some cases, basic compounds can contain counter ions (such as trifluoroacetic acid, TFA) derived from both reagent and mobile phase. These components may remain as impurities, affecting the final purity. With Nexera UFPLC, the target component can be recovered as a high-purity free base (free base type) by using a trap column.
Open Solution Software for Preparative Systems
Open Solution is open access software that not only streamlines preparative purification operations, but also supports multi-user operation of preparative systems. Even inexperienced users can perform routine preparative operations easily with minimal efforts. The use of a network contributes to improved work efficiency.
Easy Operation, Screening Using Multiple Conditions
After logging into Open Solution, analysis can be started from a single screen by simply selecting a pre-registered method and registering a sample. Screening analysis can be easily performed with the same procedure. While the system is performing an analysis, a different user can schedule the next analysis. Open Solution software will automatically include washing steps between different user methods, reducing the system downtime.
Confirmation of Fractionation and Re-injection Analysis
By selecting the vial displayed in the fraction collector diagram, the chromatogram, mass spectrum, and UV spectrum of that fraction can be easily confirmed. From the same screen, it is possible to directly check the purity of the fraction.
Automatic Scale-up from Analytical Scale to Preparative Scale
When screening analysis is performed to examine preparative conditions, the results are judged in three steps according to the degree of separation and MS spectral purity. If the judgment is acceptable, a preparative method is automatically generated. Therefore, the user can focus on examining conditions for samples with insufficient separation or aren’t detected.
