Aminoglycosides are an antibiotic family widely used for the treatment of bacterial infections in cattle, sheep, pigs and poultry. Regulatory agencies have set maximum residue limits (MRL) for these compounds after veterinary use. Aminoglycosides are very polar compounds, and usually require the use of undesirable ion-pairing reagents with LC-MS/MS.
This Method Package is a complete solution for the simultaneous quantitative analysis of all regulated aminoglycosides by HILIC/MS/MS. It includes:
Complete protocol includes step-by-step instructions for sample and calibration curve preparation.
Perform high-sensitivity analysis with excellent chromatography without resorting to ion-pairing reagents that foul the mass spectrometer. LabSolutions Insight provides a fast and intuitive environment for data processing and review. User-configurable flags alert you what values are outside of QC limits, facilitating review by exception.
MRM Spectrum Mode acquires up to 15 MRMs per analyte, and each transition is optimized to a specific collision energy. Because MS duty cycle is not wasted on meaningless portions of the spectrum, information-rich spectra can be acquired in just milliseconds. The result is an unambiguous spectrum, even for trace-level components.